Retrospective Evaluation Of Sars-Cov-2 In A Riverine Community Of Niger Delta, Nigeria Using Real-Time Polymerase Chain Reaction

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Published: Jul 1, 2024
DOI: https://doi.org/10.60787/ajrmhs-v2i1-29
Keywords:
Prevalence, SARS-CoV-2, Infection, Niger Delta, RT-Polymerase Chain Reaction

Main Article Content

Aaron U. Umasoye
Prof. Kennedy Wariso
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F. Emuh
UPTH/NCDC Molecular Laboratory, Department of Medical Microbiology, University of Port Harcourt Teaching Hospital, Port Harcourt.
B. A. Dappa
UPTH/NCDC Molecular Laboratory, Department of Medical Microbiology, University of Port Harcourt Teaching Hospital, Port Harcourt.
N. Wali
UPTH/NCDC Molecular Laboratory, Department of Medical Microbiology, University of Port Harcourt Teaching Hospital, Port Harcourt.
E. Nwachukwu
UPTH/NCDC Molecular Laboratory, Department of Medical Microbiology, University of Port Harcourt Teaching Hospital, Port Harcourt
G. F. Karibo
UPTH/NCDC Molecular Laboratory, Department of Medical Microbiology, University of Port Harcourt Teaching Hospital, Port Harcourt.
A. G. Affia
Department of Medical Laboratory Science, College of Health Science, University of Port Harcourt.
C. Ezi
Department of Medical Microbiology & Parasitology, University of Port Harcourt, Port Harcourt.
E. G. MacFiberesima
Department of Medical Microbiology & Parasitology, University of Port Harcourt, Port Harcourt.
A. T. O. Awopeju
Department of Medical Microbiology & Parasitology, University of Port Harcourt, Port Harcourt.
M. A. Alex-Wele
UPTH/NCDC Molecular Laboratory, Department of Medical Microbiology, University of Port Harcourt Teaching Hospital, Port Harcourt.
O. K. Obunge
Department of Medical Microbiology & Parasitology, University of Port Harcourt, Port Harcourt.
D. D. Alasia
COVID-19 Treatment Centre, University of Port Harcourt Teaching Hospital, Port Harcourt.
O. Maduka
COVID-19 Treatment Centre, University of Port Harcourt Teaching Hospital, Port Harcourt.

Abstract

Introduction: Global health systems are battling with the changing COVID 19 prevalence globally due to a diversity of factors. This study was carried out in a riverine community in the Niger Delta to determine the Prevalence of SARS nCov 2.


Materials and Methods: The samples were collected aseptically from the suspected patients into a virus transport medium and sent to the molecular laboratory in the cold chain for processing. After inactivation and RNA extraction procedures, all RNA extracts in the Eppendorf tube stored at -20oC were used as a template for PCR amplification with the Life-River Master-Mix procedure according to the manufacturer's instructions.


Results: This study involved 899 subjects, both male (508) and female (391), in the ratio of 1.3:1. Age group prevalence of males in this study revealed age group 31-40 years (3.23%) as the highest while male prevalence was 11.7% (105/899) and female was Prevalence 8.5% (76/899).


Conclusion: This study showed a moderate infection rate of COVID 19 among the population of people living in Yenagoa and calls for utmost preventive action to be strictly adhered to by the populace.

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How to Cite
Umasoye, A. U., Wariso, P. K., Emuh, F., Dappa, B. A., Wali, N., Nwachukwu, E., Karibo, G. F., Affia, A. G., Ezi, C., MacFiberesima, E. G., Awopeju, A. T. O., Alex-Wele, M. A., Obunge, O. K., Alasia, D. D., & Maduka, O. (2024). Retrospective Evaluation Of Sars-Cov-2 In A Riverine Community Of Niger Delta, Nigeria Using Real-Time Polymerase Chain Reaction. African Journal of Research in Medical and Health Sciences, 2(1), 45-51. https://doi.org/10.60787/ajrmhs-v2i1-29
Issue
Vol. 2 No. 1 (2024)
Section
Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Author Biographies

B. A. Dappa, UPTH/NCDC Molecular Laboratory, Department of Medical Microbiology, University of Port Harcourt Teaching Hospital, Port Harcourt.

Department of Medical Laboratory Science, College of Health Science, University of Port Harcourt.

M. A. Alex-Wele, UPTH/NCDC Molecular Laboratory, Department of Medical Microbiology, University of Port Harcourt Teaching Hospital, Port Harcourt.

Department of Medical Microbiology & Parasitology, University of Port Harcourt, Port Harcourt.

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